Lysogeny by MS2 phage. Analysis of a recombinant plasmid containing MS2 RNA-like sequence
نویسندگان
چکیده
منابع مشابه
Engineering RNA phage MS2 virus-like particles for peptide display
OF DISSERTATION Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Biomedical Sciences The University of New Mexico Albuquerque, New Mexico
متن کاملCodon usage and secondary structure of MS2 phage RNA.
MS2 is an RNA bacteriophage (3569 bases). The secondary structure of the RNA has been determined, and is known to play an important role in regulating translation. Paired regions of the genome have a higher G+C content than unpaired regions. It has been suggested that this reflects selection for high G+C content to encourage pairing, but a re-analysis of the data together with computer simulati...
متن کاملRNA folding kinetics regulates translation of phage MS2 maturation gene.
The gene for the maturation protein of the single-stranded RNA coliphage MS2 is preceded by an untranslated leader of 130 nt, which folds into a cloverleaf, i.e., three stem-loop structures enclosed by a long distance interaction (LDI). This LDI prevents translation because its 3' moiety contains the Shine-Dalgarno sequence of the maturation gene. Previously, several observations suggested that...
متن کاملA thermodynamic analysis of the sequence-specific binding of RNA by bacteriophage MS2 coat protein.
Most mutations in the sequence of the RNA hairpin that specifically binds MS2 coat protein either reduce the binding affinity or have no effect. However, one RNA mutation, a uracil to cytosine change in the loop, has the unusual property of increasing the binding affinity to the protein by nearly 100-fold. Guided by the structure of the protein-RNA complex, we used a series of protein mutations...
متن کاملReplication of Viral Rna. Iv. Properties of Rna Synthetase and Enzymatic Synthesis of Ms2 Phage Rna.
Evidence has been presented' for the accumulation of a double-stranded replicative form of MS2 RNA in vivo, following infection of Escherichia coli with MS2 phage, and for the identity of this material with the double-stranded RNA which is labeled in vitro with radioactive carbon2 on incubation of CL4-labeled ribonucleoside triphosphates with RNA synthetase.3 The present paper is concerned with...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1995
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0003d4